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An ADVANCE low flow Michrom source (Bruker-Michrom) was used to ionize peptides as they elute from the capillary column in a linear gradient from 100% buffer A (2% acetonitrile in water containing 0.1% formic acid) to 15% buffer A plus 85% buffer B (100% acetonitrile in water containing 0.1% formic acid) over 80 min

A medida que las clulas envejecen, se vuelven senescentes: menos funcionales y ms proinflamatorias

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171 Treatment with ghrelin does not affect insulin secretion at 5.5mM glucose but significantly reduces it (by ~30%) at 16.8mM glucose